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	Provedor de dados BJM (2) Autor Adnan,Ahmad (2) Ahmad,Waqar (2) Shabbiri,Khadija (2) Jamil,Sania (1) Noor,Bushra (1) Rafique,H.M. (1) Syed,Quratulain (1) Mais... Palavra-chave Brevibacterium linens (1) Central Composite Design (CCD) (1) Complex II (1) Cytochrome b (1) Plackett-Burman Design (1) Protease (1) Proteus mirabilis (1) Solid-state fermentation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 425 Primeira ... 4 5 6 7 8 9 10 11 12 ... Última Detection of fumonisin producing Fusarium verticillioides in paddy (Oryza sativa L.) using polymerase chain reaction (PCR) BJM Maheshwar,P.K.; Moharram,S. Ahmed; Janardhana,G.R.. The study reports the occurrence of fumonisin producing Fusarium verticillioides in 90 samples of stored paddy (Oryza sativa L.)collected from different geographical regions ofKarnataka, India. Fumonisin producing F. verticillioides was identified based on micromorphological characteristics and PCR using two sets of primers. One set of primers was F. verticillioides species specific, which selectively amplified the intergenic space region of rDNA. The other set of primers was specific to fumonisin producing F. verticillioides. Eight paddy samples were positive for F. verticillioides. Eleven isolates obtained from these samples were capable of producing fumonisin. Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium verticillioides; Fumonisin; Paddy; PCR. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100023 Detection of Genetic Relationship in the Tree of Life of Some Quail Through Molecular Markers Analyses BABT Deef,Lamiaa Elsayed Mokhtar. ABSTRACT Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and the Bobwhite quail (Colinus virginianus) were used in this study. PCR-RFLP and SDS-proteins were performed to reveal the genetic characterization and genetic relationship of the studied quails. Analysis of fragments generated by digestion of PCR product with restriction enzyme NlaIII recorded highly polymorphic restriction profiles. There is a wide intraspecific COI, SEMA3E and TLX genes variability among the studied quails. Protein bands varied from10 to 18 between quails with minimum number of bands were in the Dotted... Tipo: Info:eu-repo/semantics/article Palavras-chave: Birds; PCR; Quail; SDS-PAGE; Galliformes. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100425 Detection of HCV by PCR in serum and PBMC of patients with hepatitis C after treatment BJID Cavalheiro,Norma de Paula; Filgueiras,Thelma Cristina; Melo,Carlos Eduardo; Morimitsu,Suzana Rie; Araújo,Evaldo Stanislau Affonso de; Tengan,Fátima Mitiko; Barone,Antonio Alci. Although hepatitis C is mainly hepatotropic, some studies suggest that hepatitis C virus (HCV) infects peripheral blood mononuclear cells (PBMC), using them as a reservoir, which might contribute to the development of resistance to treatment. Fifty-four hepatitis-C patients, who had been submitted to treatment, were selected. Blood samples were collected on the same day for the detection of HCV RNA in serum and PBMC by PCR, using the Amplicor HCV 2.0 assay (Roche Diagnostics). HCV genotyping was performed using the INNO-LiPA HCV kit (Versant, Bayer Diagnostics). HCV RNA was detected in both serum and PBMC in 35 (64%) patients and no RNA in 16 (29.6%). Disagreement between the serum and PBMC results was observed for three patients (5.6%), with HCV RNA being... Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR; Hepatitis C; PBMC; Treatment. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000500006 Detection of hepatitis B virus A1762T/G1764A mutant by amplification refractory mutation system BJID Wang,Yong-Zhong; Zhu,Zhen; Zhang,Hong-Yu; Zhu,Min-Zhi; Xu,Xin; Chen,Chun-Hua; Liu,Long-Gen. OBJECTIVE: To study the role of hepatitis B virus with A1762T/G1764A double mutation in liver cirrhosis and hepatocellular carcinoma, and create a sensitive, fast, accurate assay for detection of A1762T/G1764A double mutation.METHODS: We developed an accurate and fast real-time amplification refractory mutation system to detect A1762T/G1764A double mutation. Cloned hepatitis B virus genome was used as a control. Assay sensitivity was determined by serial dilution and mixed template experiments. Specificity was determined by cross experiments with wild and mutant hepatitis B virus. Fifty clinical samples were tested by the real-time amplification refractory mutation system and the results were compared with sequencing.RESULTS: The real-time amplification... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepatitis B virus; Basal core promoter mutations; Amplification refractory mutation system; PCR. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300261 Detection of Leptospira spp. in the semen and urine of bulls serologically reactive to Leptospira interrogans serovar hardjo BJM Magajevski,Fernanda Senter; Girio,Raul Jose Silva; Mathias,Luis Antonio; Myashiro,Simone; Genovez,Margareth Élide; Scarcelli,Eliana Pinheiro. This study evaluated PCR for the detection of leptospires in semen and urine of ten serologically reactive bulls, comparing these results with those obtained with other diagnosis techniques. Two collections of materials were done in alternate days. Semen and urine samples were separated in aliquots for: direct visualization in dark field microscopy; inoculation in hamsters (for semen only); isolation in culture media; and PCR. No hamster was positive by the microscopic agglutination test (MAT); kidney and liver fragments from the hamsters were used in an isolation attempt in culture media, with one positive isolation from the kidney of a hamster inoculated with semen of one bull, and from liver of hamsters inoculated with semen of three bulls. Isolation in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Semen; Urine; PCR. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100009 Detection of Lsr2 gene of Mycobacterium leprae in nasal mucus BABT Custodio,Luiz Antonio; Saito,Alexandre; Amarante,Marla Karine; Fujita,Thiago Cezar; Perim,Aparecida de Lourdes; Costa,Ivete Conchon; Felipe,Ionice; Jankevicius,Shiduca Itow. In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific primers for Lsr2 gene of Mycobacterium leprae. The presence of Lsr2 gene in the nasal mucus was detected in 25.80% of patients with paucibacillari leprosy, and 23.07% of contacts. Despite the absence of clinical features in the contact individuals, it was possible to detect the presence of Lsr2 gene in the nasal mucus of these individuals. Therefore, PCR detection of M. leprae targeting Lsr2 gene using nasal mucus samples could contribute to early diagnosis of leprosy. Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium leprae; Leprosy; Hanseniase; Nasal mucus; PCR. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300007 Detection of mutations within exons 4 to 8 of the p53 tumor suppressor gene in canine mammary glands Arq. Bras. Med. Vet. Zootec. Souza,D.M.B.; Barros,M.G.O.; Silva,J.S.C.; Silva,M.B.; Coleto,Z.F.; Jimenez,G.C.; Adrião,M.; Wischral,A.. Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bitch; DNA; Mamma; Molecular biology; Neoplasm; PCR. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013 Detection of Mycoplasma gallisepticum in dead captive psittacines in Belo Horizonte, Brazil Rev. Bras. Ciênc. Avic. Gomes,AM; Costa,LL; Vilela,DAR; Marques,MVR; Carvalhaes,AG; Marin,SY; Costa,MP; Horta,RS; Resende,JS; Martins,NRS. Mycoplasma gallisepticum (Mg) infection of wild native Brazilian psittacines (Psittaciformes) which died of any cause during sorting, rehabilitation, or conservation, was investigated by PCR. Two previously described PCR methodologies using Mg specific primers were employed for the analyses of 140 swab samples (cloaca, trachea, or palatine cleft). Average positive Mg detection in cloacal swabs was 51.9%, with 80.0% (n=5) of Blue-and-yellow Macaws (Ara ararauna), 60.0% (n=3) Dusky Parrots (Pionus fuscus), 52.5% (n=59) Amazon Parrots (Amazona aestiva), 50.0% (n=2) Orange-winged Parrots (Amazona amazonica), 50.0% (n=2) Jandaya Parakeetsor Jandaya Conures (Aratinga jandaya), 0% (n=2) Golden Conures or Golden Parakeets (Guarouba guarouba), and 0% (n=2) Hyacinth... Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazona aestiva; Amazona amazonica; Anodorhynchus hyacinthinus; Aratinga jandaya; Brazilian psittacine fauna; Guarouba guarouba; Mycoplasma gallisepticum; PCR; Pionus fuscus; Psittaciformes; Psittacidae. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200001 Detection of oral streptococci in dental biofilm from caries-active and caries-free children BJM Silva,Andréa Cristina Barbosa da; Cruz,Jader dos Santos; Sampaio,Fábio Correia; Araújo,Demetrius Antônio Machado de. This work correlated the presence of oral streptococci in dental biofilm with clinical indexes of caries and oral hygiene in caries-active and caries-free children. S. mutans and/or S. sobrinus in the dental biofilm does not indicate a direct risk for developing dental caries. Tipo: Info:eu-repo/semantics/article Palavras-chave: Dental caries; Dental biofilm; Oral streptococci; PCR; Glucosyltranferases. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400009 Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: A critical review ArchiMer Batista, Frederico; Arzul, Isabelle; Pepin, Jean-francois; Ruano, Francisco; Friedman, Carolyn S.; Boudry, Pierre; Renault, Tristan. Herpes-like viral infections have been reported in different bivalve mollusc species throughout the world. High mortalities among hatchery-reared larvae and juveniles of different bivalve species have been associated often with such infections. The diagnosis of herpes-like viruses in bivalve molluscs has been performed traditionally by light and transmission electron microscopy. The genome sequencing of one of these viruses, oyster herpesvirus 1 (OsHV-1), allowed the development of DNA-based diagnostic techniques. The polymerase chain reaction (PCR) has been used for the detection of OsHV-1 DNA in bivalve molluscs at different development stages. In addition, the PCR used for detection of OsHV-1 has also allowed the amplification of DNA from an OsHV-1... Tipo: Text Palavras-chave: Oyster; Bivalve molluscs; PCR; Detection; OsHV 1; Herpesvirus. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2322.pdf Detection of ostreid herpesvirus 1 (OsHV-1) DNA in seawater by PCR: influence of water parameters in bioassays ArchiMer Vigneron, Vassilia; Solliec, Gaelle; Montanie, Hélène; Renault, Tristan. Since 1991, herpesvirus infections have been reported among larvae and juveniles of various bivalves. Most of the studies focused on detection of viral infections of economically important species. However, the persistence of bivalve herpesviruses; in the marine environment is poorly documented. The present study concerns the role of seawater parameters in Ostreid Herpesvirus I (OsHV-1) detection by polymerase chain reaction (PCR). Viral DNA extracted from purified particles or virions present in infected oyster larvae were detected by PCR after storage in different media at different temperatures. The lowest detection threshold was found using distilled water or Tris EDTA buffer. In seawater, the threshold was higher. The use of sterile media permitted... Tipo: Text Palavras-chave: Temperature; PCR; Detection; Seawater; Viral DNA; Oyster herpesvirus 1. Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-2909.pdf Detection of papillomavirus DNA in formalin-fixed paraffin-embedded equine aural plaque samples Arq. Bras. Med. Vet. Zootec. Zakia,L.S.; Basso,R.M.; Olivo,G.; Herman,M.; Araujo Jr.,J.P.; Borges,A.S.; Oliveira-Filho,J.P.. A placa aural é uma dermatopatia associada à quatro Equus caballus papillomavirus (EcPVs). Até o momento, o DNA de EcPVs não foi identificado em amostras de placa aural fixadas em formalina e embebidas em parafina (FFPE). O objetivo deste estudo foi otimizar um método para a detecção dos quatro tipos de EcPVs em 21 amostras FFPE usando a PCR. O DNA dos EcPVs foram detectados em 11 amostras (52.4%). O DNA do EcPV4 foi detectado em 38.1% (8/21) e do EcPV3 em 4.8% (1/21) das amostras. Coinfecção foi identificada em duas amostras (9.5%); EcPV4 e 5 foram detectados simultaneamente em uma amostra, enquanto o DNA dos EcPV4 e 6 foi detectado em outra. A especificidade do DNA dos papilomavírus equinos foi avaliada por sequenciamento gênico direto, que confirmou a... Tipo: Info:eu-repo/semantics/other Palavras-chave: Horses; Formalin-fixed and paraffin-embedded samples; Papillomaviruses; PCR. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000401193 Detection of point mutations by non-isotopic single strand conformation polymorphism BJMBR Pinheiro,N.A.; Moura,R.P.; Monteiro,E.; Villa,L.L.. We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplification of DNA fragments by PCR with specific oligonucleotide primers, denaturation, and electrophoresis on small neutral polyacrylamide gels, followed by silver staining. The sensitivity of this procedure is comparable to other described techniques and the method is easy to perform and applicable to a variety of tissue specimens. Tipo: Info:eu-repo/semantics/other Palavras-chave: P53 gene; Human papillomavirus; PCR; SSCP; Cancer; Silver staining. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100008 Detection of Rhynchosporium secalis in barley seeds from Argentina through polymerase chain reaction technique Trop. Plant Pathol. Ríos,Melina Ocampo; Fernández,Paula; Carmona,Marcelo. Leaf scald of barley caused by Rhynchosporium secalis is an important disease in Argentina. The fungus is a necrotrophic pathogen which survives in stubble, seeds and weeds. Isolation of R. secalis from seeds on artificial media usually has not been successful due to the slow growth rate of the pathogen and strong inhibition by contaminants. The objective in this work was to detect R. secalis in different genotypes of barley seeds in Argentina using the polymerase chain reaction (PCR)-based diagnostic assay. Four barley genotypes were tested in 2004: Quilmes Ayelén, Quilmes Alfa, Barke and Maltería Pampa 1004. The previously described RS8 and RS9 primers were used for the detection of R. secalis in barley seeds. A 264-bp single band was obtained for each... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hordeum vulgare; Diagnosis; PCR. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000500007 Detection of Salmonella sp. from porcine origin: a comparison between a PCR method and standard microbiological techniques BJM Castagna,Sandra Maria Ferraz; Muller,Monika; Macagnan,Marisa; Rodenbusch,Carla Rosane; Canal,Cláudio Wageck; Cardoso,Marisa. The aim of this study was to compare a polymerase chain reaction (PCR) method combined with selective enrichment in Rappaport-Vassiliadis broth (PCR-RVB) with standard microbiological techniques (SMT) for the generic detection of Salmonella in samples of porcine origin. Two hundred sixty eight field samples consisting of 42 sets of pooled porcine mandibular lymph nodes and tonsils, 44 samples of intestinal content, 38 pork sausage meat samples and 144 samples of feed collected from swine farms were submitted to the PCR-RVB and SMT protocols. Salmonella was detected in 54 samples using the PCR-RVB assay and in 42 samples by SMT, three of the SMT Salmonella-positive samples (one each of S. Derby, S. Panama and S. Typhimurium) being Salmonella-negative by... Tipo: Info:eu-repo/semantics/article Palavras-chave: Detection; PCR; Pork; Salmonella; Swine. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400013 Detection of shigella in lettuce by the use of a rapid molecular assay with increased sensitivity BJM Jiménez,Kenia Barrantes; McCoy,Clyde B.; Achí,Rosario. A Multiplex Polymerase Chain Reaction (PCR) assay to be used as an alternative to the conventional culture method in detecting Shigella and enteroinvasive Escherichia coli (EIEC) virulence genes ipaH and ial in lettuce was developed. Efficacy and rapidity of the molecular method were determined as compared to the conventional culture. Lettuce samples were inoculated with different Shigella flexneri concentrations (from 10 CFU/ml to 10(7) CFU/ml). DNA was extracted directly from lettuce after inoculation (direct-PCR) and after an enrichment step (enrichment PCR). Multiplex PCR detection limit was 10(4) CFU/ml, diagnostic sensitivity and specificity were 100% accurate. An internal amplification control (IAC) of 100 bp was used in order to avoid false... Tipo: Info:eu-repo/semantics/article Palavras-chave: PCR; Shigella; Rapid method; Lettuce; Food. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000400018 Detection of Toxoplasma gondii by PCR and mouse bioassay in commercial cuts of pork from experimentally infected pigs Arq. Bras. Med. Vet. Zootec. Tsutsui,V.S.; Freire,R.L.; Garcia,J.L.; Gennari,S.M.; Vieira,D.P.; Marana,E.R.M.; Prudêncio,L.B.; Navarro,I.T.. The distribution of T. gondii in commercial cuts of pork (ham, tenderloin, spareribs and arm picnic) by PCR and bioassay from experimentally infected pigs, was evaluated. Eighteen mixed breed pigs were divided into two groups (G). The G1 animals (n=10) were infected with 4 x10(4) oocysts of the T. gondii VEG strain and the G2 animals (n=8) were used as control. Pigs of both groups were slaughtered at 59th day after infection, and meat samples were collected for bioassay and PCR. All animals from G1 were positive by at least one or both tests, and all control animals were negative. T. gondii was identified in pork by mouse bioassay and PCR in 27/40 (67.5%) and in 9/40 (22.5%) of the evaluated samples, respectively. There were no statistical differences in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pork meat; Mouse bioassay; PCR; Toxoplasma gondii. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000100006 Detection of Toxoplasma gondii DNA in sera samples of mice experimentally infected J. Venom. Anim. Toxins incl. Trop. Dis. Langoni,H.; Das Dores,C. B.; Silva,R. C.; Pezerico,S. B.; Castro,A. P. B.; Da Silva,A. V.. Detection of Toxoplasma gondii (T. gondii) DNA in blood can help to diagnose the disease in its acute phase; however, it must be considered that hemoglobin, present in blood, can inhibit polymerase activity, making impracticable the detection of DNA in samples. Mice were experimentally infected via oral route with ME49 and BTU2 strains cysts and RH strain tachyzoites; polymerase chain reaction was used to detect T. gondii DNA in mice sera 18, 24, 48, 96, and 192 hours post infection (PI). Toxoplama gondii DNA was detected in only one animal infected with BTU2 strain, genotype III (isolated from a dog with neurological signs) 18 hours PI. The agent's DNA was not detected in any sample of the other experimental groups. New studies must be carried out to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasmosis; PCR; Serum; Experimental infection; Mice. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200004 Detection of Toxoplasma gondii in a free-ranging giant anteater Ciência Rural Morgado,Thais Oliveira; Kagueyama,Francielle Cristina; Rosa,Janaina Marcela Assunção; Belizário,Melissa Debesa; Pacheco,Richard de Campos; Dutra,Valéria; Corrêa,Sandra Helena Ramiro; Paz,Regina Celia Rodrigues da. ABSTRACT: Toxoplasmosis is caused by Toxoplasma gondii, an obligatory intracellular protozoan, which establishes acute and chronic infections in birds and mammals, including humans. This note reports, for the first time, the detection and sequencing of DNA from T. gondii in the peripheral blood of a young free range giant anteater (Myrmecophaga tridactyla). For the diagnosis, the following methods were used: polymerase chain reaction (PCR) and positive serology (1:800) by means of the modified agglutination test (MAT). Since this species may be consumed by humans and predated by wild felids, its importance is emphasized as a probable source of zoonotic infection, in addition to its possible participation in the infection enzootic cycle. Although,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Myrmecophaga tridactyla; PCR; Modified agglutination test; Blood. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800501 Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats J. Venom. Anim. Toxins incl. Trop. Dis. Costa,VM; Langoni,H. The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR. Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxoplasma gondii; Parasite in milk; Female rats; PCR; Bioassay. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200016 Registros recuperados: 425 Primeira ... 4 5 6 7 8 9 10 11 12 ... Última

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